Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA300858

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

19/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

miRNA-seq (Phytophthora sojae) with severe quality failure (19/100, grade F). Q30 at 59.8% and mean base quality of 24.8 both score zero—far below thresholds for confident miRNA quantification. The 69% duplication and older GASeq II platform compound the failure; resequencing on a modern platform is necessary.

Data type / assay
bulk-RNA-seq
Organism
Phytophthora sojae
Instrument
Illumina Genome Analyzer II
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
47 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 91382034138 reported
total reads 1776458246 reported
n content pct 0.028 measured
pct q20 bases 72.2 measured
pct q30 bases 59.8 measured
gc content pct 39.2 measured
mean read length 76 measured
mean base quality 24.8 measured
adapter content pct 0 measured
duplication rate pct 69.26 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 19/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 59.8 measured ×1 0%
mean base quality 24.8 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 69.26 measured ×0.4 13%
QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0