Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
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PRJNA302180

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Amplicon (Illumina MiSeq, freshwater) with poor quality (33/100, grade F). Q30 at 66.3% (scoring zero) is below the ~85% benchmark, severely limiting taxonomic confidence, and 90.22% duplication indicates over-amplification or limited input. Data should not be reused; obtain fresh samples or alternative source.

Data type / assay
amplicon
Organism
freshwater metagenome
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
77 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1029568917 reported
total reads 1827241 reported
n content pct 0 measured
pct q20 bases 80.6 measured
pct q30 bases 66.3 measured
gc content pct 52.8 measured
mean read length 285 measured
mean base quality 30.2 measured
adapter content pct 0 measured
duplication rate pct 90.22 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 66.3 measured ×1 0%
adapter content pct 0 measured ×0.5 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0