PRJNA317653

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human gut amplicon metagenome of C grade; pct_q30_bases (83.8%, measured) is below A-tier and combined with elevated duplication rate (69.23%, measured) may inflate abundance estimates of common taxa while losing rare variants. Measured evidence_strength=1 confirms this is not provisional; usable for robust OTU composition but not for sensitive ecological analysis.

Data type / assay

amplicon

Organism

human gut metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

32 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 238684713 reported

total reads 949351 reported

n content pct 0 measured

pct q20 bases 86.9 measured

pct q30 bases 83.8 measured

gc content pct 33.2 measured

mean read length 108.3 measured

mean base quality 33.6 measured

adapter content pct 0 measured

duplication rate pct 69.23 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.8 measured ×1 69%

adapter content pct 0 measured ×0.5 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0