Corpus 1,276 assessed · 1,177 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA323602

BioProject first seen 2024

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

MiSeq amplicon sequencing of freshwater metagenome with 29.2 million short reads and 14.6 Gb at moderate quality (90.6% Q20, 85% Q30). Comprehensive freshwater microbiota profiling; lower Q-scores may compromise rare OTU assignments in degraded samples.

Data type / assay
amplicon
Organism
freshwater metagenome
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
201 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 14623143828 reported
total reads 29170953 reported
n content pct 0.017 measured
pct q20 bases 90.6 measured
pct q30 bases 85 measured
gc content pct 52.9 measured
mean read length 250.6 measured
mean base quality 34 measured
adapter content pct 0 measured
duplication rate pct 96.76 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85 measured ×1 75%
adapter content pct 0 measured ×0.5 100%
QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0