Corpus 1,275 assessed · 1,176 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74.1/100
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PRJNA33179

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

45/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This 454 GS FLX amplicon dataset from an environmental metagenome contains 9.4 million reads with 73.6% Q30 bases, serving as a legacy dataset for method benchmarking and historical microbiome platform comparison. Researchers validating bioinformatic pipelines or studying 454 sequencing characteristics will find data demonstrating platform-specific read patterns and quality profiles. The lower quality scores require stringent filtering before OTU/ASV clustering and taxonomic assignment.

Data type / assay
amplicon
Organism
metagenome
Instrument
454 GS FLX
Platform
CAPILLARY
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
37 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 5368630731 reported
total reads 9434470 reported
n content pct 0.014 measured
pct q20 bases 90.1 measured
pct q30 bases 73.6 measured
gc content pct 39 measured
mean read length 374.3 measured
mean base quality 33.6 measured
adapter content pct 0 measured
duplication rate pct 1.38 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 45/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 73.6 measured ×1 18%
adapter content pct 0 measured ×0.5 100%
QC cost 37 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0