PRJNA341855

BioProject first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

72/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Graphocephala coccinea (red-headed leafhopper) RNA-seq from an Illumina HiSeq 2500 with 319 million short reads at 81% Q30. The large transcriptome enables insect vector genomics and identification of pathogen-interacting genes.

Data type / assay

bulk-RNA-seq

Organism

Graphocephala coccinea

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

8 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 82555473037 reported

total reads 319671748 reported

n content pct 0.125 measured

pct q20 bases 87.8 measured

pct q30 bases 81.3 measured

gc content pct 41.9 measured

mean read length 151 measured

mean base quality 34.5 measured

adapter content pct 0.98 measured

duplication rate pct 55.75 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 72/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.3 measured ×1 56%

mean base quality 34.5 measured ×0.6 100%

adapter content pct 0.98 measured ×0.4 100%

duplication rate pct 55.75 measured ×0.4 43%

QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0