PRJNA344791

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

34/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon of human gut (MiSeq, 300.8bp). Fails QC: only 70.1% Q30 (score 0/100) and mean quality 31.1 are insufficient; duplication 91.25% masks true community structure. All metrics measured. Unsuitable without intensive filtering.

Data type / assay

amplicon

Organism

human gut metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

43 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 8005738984 reported

total reads 13324740 reported

n content pct 0.001 measured

pct q20 bases 84.8 measured

pct q30 bases 70.1 measured

gc content pct 53.8 measured

mean read length 300.8 measured

mean base quality 31.1 measured

adapter content pct 0 measured

duplication rate pct 91.25 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 34/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 70.1 measured ×1 1%

adapter content pct 0 measured ×0.5 100%

QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0