PRJNA353241

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

NextSeq 500 bulk RNA-Seq of Pseudomonas aeruginosa with 132 million short reads and 20 Gb at high quality (96.2% Q20, 94.3% Q30, 63.5% GC). Enables virulence factor expression profiling and condition-specific transcriptomics in this opportunistic pathogen.

Data type / assay

bulk-RNA-seq

Organism

Pseudomonas aeruginosa

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

18 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 20129132912 reported

total reads 132428506 reported

n content pct 0.001 measured

pct q20 bases 96.2 measured

pct q30 bases 94.3 measured

gc content pct 63.5 measured

mean read length 76 measured

mean base quality 34.4 measured

adapter content pct 0.21 measured

duplication rate pct 44.3 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.3 measured ×1 100%

mean base quality 34.4 measured ×0.6 100%

adapter content pct 0.21 measured ×0.4 100%

duplication rate pct 44.3 measured ×0.4 68%

QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0