Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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PRJNA353588

BioProject

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Large-scale human RNA-seq on HiSeq 2500 with 13.4 billion 101 bp reads (1.35 Tb) at 94.9% Q20 and 91.4% Q30 provides massive depth for transcriptome analysis. The 41.2% GC and reasonable quality enable sensitive detection of isoforms, allele-specific expression, and rare transcripts across diverse human tissues and conditions.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
N numbers (samples, groups)
826 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1353427425853 reported
total reads 13407913328 reported
n content pct 0.005 measured
pct q20 bases 94.9 measured
pct q30 bases 91.4 measured
gc content pct 41.2 measured
mean read length 101 measured
mean base quality 35.8 measured
adapter content pct 0.05 measured
duplication rate pct 44.97 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.4 measured ×1 100%
mean base quality 35.8 measured ×0.6 100%
adapter content pct 0.05 measured ×0.4 100%
duplication rate pct 44.97 measured ×0.4 67%
QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 84