PRJNA353903

BioProject first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

87/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read RNA-seq of fungal pathogen. Base quality is strong (96.2% Q30) but elevated adapter (5.04%, score 83/100) and high duplication (56.22%, score 42/100) indicate library or PCR issues. All metrics measured. Usable with caution.

Data type / assay

bulk-RNA-seq

Organism

Talaromyces marneffei PM1

Instrument

Illumina HiSeq 1500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

8 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 55500844916 reported

total reads 274756658 reported

n content pct 0.001 measured

pct q20 bases 98.3 measured

pct q30 bases 96.2 measured

gc content pct 46.3 measured

mean read length 101 measured

mean base quality 36.1 measured

adapter content pct 5.04 measured

duplication rate pct 56.22 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 87/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.2 measured ×1 100%

mean base quality 36.1 measured ×0.6 100%

adapter content pct 5.04 measured ×0.4 83%

duplication rate pct 56.22 measured ×0.4 42%

QC cost 37 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0