Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA360482

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

46/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Cotton (Gossypium barbadense) bulk RNA-seq on HiSeq 2000 spans 126 billion bases (739 million reads) with substantially lower quality (71% Q30 bases), suggesting potential platform or sample degradation issues. The large volume of sequence data enables broad transcript discovery despite quality concerns, but variant calling and splice-site detection would require stringent filtering; best suited to transcript assembly and abundance estimates.

Data type / assay
bulk-RNA-seq
Organism
Gossypium barbadense
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 125851596973 reported
total reads 739437386 reported
n content pct 0.014 measured
pct q20 bases 89.6 measured
pct q30 bases 71 measured
gc content pct 49.6 measured
mean read length 90 measured
mean base quality 30.6 measured
adapter content pct 0 measured
duplication rate pct 28.56 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 46/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 71 measured ×1 5%
mean base quality 30.6 measured ×0.6 43%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 28.56 measured ×0.4 100%
QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0