PRJNA369179

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read amplicon of human gut (MiSeq, 271bp). Fails QC: only 56% Q30 bases (score 0/100) and mean quality 27.3 are critically low; duplication 89.08% masks true diversity. All metrics measured. Not suitable for reliable taxonomy without extensive filtering.

Data type / assay

amplicon

Organism

gut metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

16 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 307982468 reported

total reads 571587 reported

n content pct 0.157 measured

pct q20 bases 70.4 measured

pct q30 bases 56 measured

gc content pct 55.3 measured

mean read length 271 measured

mean base quality 27.3 measured

adapter content pct 0 measured

duplication rate pct 89.08 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 56 measured ×1 0%

adapter content pct 0 measured ×0.5 100%

QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0