Corpus 1,276 assessed · 1,177 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA378448

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

71/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Cotton bollworm (Helicoverpa armigera) bulk RNA-seq on the older Illumina Genome Analyzer IIx yields 400 billion bases (1.8 billion reads) with moderate quality (84.3% Q30 bases), providing extensive transcriptomic data. The large volume supports isoform discovery and annotation in this pest insect, though older instrument chemistry may introduce platform-specific biases; well-suited for transcript assembly and abundance estimation.

Data type / assay
bulk-RNA-seq
Organism
Helicoverpa armigera
Instrument
Illumina Genome Analyzer IIx
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
67 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 400094452904 reported
total reads 1819118667 reported
n content pct 0.991 measured
pct q20 bases 91.1 measured
pct q30 bases 84.3 measured
gc content pct 51.2 measured
mean read length 101 measured
mean base quality 33.1 measured
adapter content pct 12.67 measured
duplication rate pct 38.53 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 71/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.3 measured ×1 72%
mean base quality 33.1 measured ×0.6 85%
adapter content pct 12.67 measured ×0.4 41%
duplication rate pct 38.53 measured ×0.4 81%
QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0