Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA395283

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

HiSeq 2500 bulk RNA-Seq of Suaeda salsa (halophyte) with 195 million short reads and 39.4 Gb at very poor quality (3.6% Q20, 1.3% Q30, 0.201% N content). Severe systematic quality degradation indicates library or sequencing issues; data likely unsuitable without extensive validation.

Data type / assay
bulk-RNA-seq
Organism
Suaeda salsa
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
8 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 39361260160 reported
total reads 194869989 reported
n content pct 0.201 measured
pct q20 bases 3.6 measured
pct q30 bases 1.3 measured
gc content pct 48 measured
mean read length 101 measured
mean base quality 3.1 measured
adapter content pct 0 measured
duplication rate pct 0.97 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 1.3 measured ×1 0%
mean base quality 3.1 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 0.97 measured ×0.4 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0