PRJNA396064

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Short-read WGS of human metagenome (MiSeq). Critically high duplication (55.61%, score 0/100) indicates PCR or library bias, severely limiting abundance quantification reliability despite acceptable Q30 (90.2%). All metrics measured. Reuse not recommended.

Data type / assay

WGS

Organism

human metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

125 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 24738882565 reported

total reads 116649290 reported

n content pct 0.23 measured

pct q20 bases 95.8 measured

pct q30 bases 90.2 measured

gc content pct 44.6 measured

mean read length 166.8 measured

mean base quality 35.4 measured

adapter content pct 0 measured

duplication rate pct 55.61 measured

How this grade was computed

Weighted mean of 3 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.2 measured ×1 100%

duplication rate pct 55.61 measured ×0.5 0%

adapter content pct 0 measured ×0.4 100%

QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0