Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA399053

BioProject

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

89/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (Locusta migratoria, HiSeq 4000) with B-grade score (89/100). Excellent base quality (Q30 97.3%, mean 39.8) and high coverage (261M reads) are tempered only by significant duplication (58.49%, scoring 37/100), which becomes the limiting factor. Reusable with duplicate-aware quantification to avoid inflation of low-abundance transcripts.

Data type / assay
bulk-RNA-seq
Organism
Locusta migratoria
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
N numbers (samples, groups)
4 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 52200203600 reported
total reads 261001018 reported
n content pct 0.005 measured
pct q20 bases 99.2 measured
pct q30 bases 97.3 measured
gc content pct 53.7 measured
mean read length 100 measured
mean base quality 39.8 measured
adapter content pct 0.6 measured
duplication rate pct 58.49 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 89/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.3 measured ×1 100%
mean base quality 39.8 measured ×0.6 100%
adapter content pct 0.6 measured ×0.4 100%
duplication rate pct 58.49 measured ×0.4 37%
QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 flagged mean score 60