Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA413627

BioProject first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

29/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

RNA-seq of the thermophilic bacterium Geobacillus stearothermophilus ATCC 12980 on MiSeq yields only 644 million bases (4.3 million reads) with lower quality (74.9% Q30 bases). The small dataset and modest quality restrict transcriptome coverage; useful primarily for discovery in a well-characterized model organism where low-abundance transcripts may be less critical.

Data type / assay
bulk-RNA-seq
Organism
Geobacillus stearothermophilus ATCC 12980
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 644120851 reported
total reads 4265701 reported
n content pct 0 measured
pct q20 bases 86.4 measured
pct q30 bases 74.9 measured
gc content pct 33.4 measured
mean read length 151 measured
mean base quality 32.2 measured
adapter content pct 96.41 measured
duplication rate pct 71.47 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 29/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 74.9 measured ×1 25%
mean base quality 32.2 measured ×0.6 70%
adapter content pct 96.41 measured ×0.4 0%
duplication rate pct 71.47 measured ×0.4 8%
QC cost 12 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0