Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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PRJNA413805

BioProject first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Escherichia coli amplicon-seq on HiSeq 4000 yields 51 billion bases (255 million reads) with excellent quality (96.6% Q30 bases), providing exceptional depth for targeted bacterial genomics. The massive amplicon dataset enables ultra-sensitive variant detection, monitoring of very low-frequency mutations, and population-level evolutionary studies in this model organism.

Data type / assay
amplicon
Organism
Escherichia coli
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
9 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 51126748600 reported
total reads 255633743 reported
n content pct 0.019 measured
pct q20 bases 98.6 measured
pct q30 bases 96.6 measured
gc content pct 45.4 measured
mean read length 100 measured
mean base quality 39.6 measured
adapter content pct 85.23 measured
duplication rate pct 25.14 measured
mean target coverage 555725.5 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.6 measured ×1 100%
adapter content pct 85.23 measured ×0.5 0%
QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0