PRJNA419242

BioProject first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing of Acanthocnemus nigricans on HiSeq 2500 delivers 28 billion bases (152 million reads) with high quality (92.7% Q30 bases), enabling confident targeted analysis of this organism. The large volume and excellent quality make the dataset suitable for population genetics or variant-frequency surveys in this insect species.

Data type / assay

amplicon

Organism

Acanthocnemus nigricans

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

375 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 28372101109 reported

total reads 152604035 reported

n content pct 0.002 measured

pct q20 bases 98.3 measured

pct q30 bases 92.7 measured

gc content pct 48.5 measured

mean read length 92.6 measured

mean base quality 35.8 measured

adapter content pct 0 measured

duplication rate pct 27.16 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.7 measured ×1 100%

adapter content pct 0 measured ×0.5 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0