Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA448331

BioProject first seen 2018

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Synthetic construct full-length cDNA sequencing via Oxford Nanopore MinION yields 4.2 billion bases (828,000 reads) with very low accuracy (5.7% Q20 bases, 0% Q30 bases), characteristic of long-read nanopore technology. The dataset enables full-transcript isoform resolution of the synthetic RNA construct, though low per-read accuracy necessitates high sequencing depth or complementary short-read polishing for consensus accuracy.

Data type / assay
bulk-RNA-seq
Organism
synthetic construct
Instrument
MinION
Platform
OXFORD_NANOPORE
Read type
long-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4157240936 reported
total reads 828684 reported
n content pct 0 measured
pct q20 bases 5.7 measured
pct q30 bases 0 measured
gc content pct 43.4 measured
mean read length 992.5 measured
mean base quality 8 measured
adapter content pct 1.55 measured
duplication rate pct 14.42 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 0 measured ×1 0%
mean base quality 8 measured ×0.6 0%
adapter content pct 1.55 measured ×0.4 100%
duplication rate pct 14.42 measured ×0.4 100%
QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0