Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA453554

BioProject

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Abalone (Haliotis fulgens) RNA-seq on MiSeq with 31 million 294.5 bp reads at 92.1% Q20 and 82.3% Q30 across 18.3 Gb provides unusually long read lengths for Illumina platforms, possibly indicating sequencing errors or PE overlaps. The 42.7% GC and moderate quality require careful alignment parameters; enables isoform-level analysis if reads are validated.

Data type / assay
bulk-RNA-seq
Organism
Haliotis fulgens
Instrument
Illumina MiSeq
Platform
ILLUMINA
N numbers (samples, groups)
2 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 18320687128 reported
total reads 31022126 reported
n content pct 0.005 measured
pct q20 bases 92.1 measured
pct q30 bases 82.3 measured
gc content pct 42.7 measured
mean read length 294.5 measured
mean base quality 33.8 measured
adapter content pct 0.21 measured
duplication rate pct 5.58 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.3 measured ×1 62%
mean base quality 33.8 measured ×0.6 97%
adapter content pct 0.21 measured ×0.4 100%
duplication rate pct 5.58 measured ×0.4 100%
QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 80