PRJNA521988

BioProject

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

0/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing of coral-associated microbial communities via Illumina MiSeq with unusually low quality metrics (55.6% Q20, 45% Q30) suggesting challenging PCR targets or library issues. Despite quality concerns, provides taxonomic profiling of host-associated microbiota; results require stringent filtering and validation.

Data type / assay

amplicon

Organism

coral metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

65 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1791813547 reported

total reads 5935912 reported

n content pct 0 measured

pct q20 bases 55.6 measured

pct q30 bases 45 measured

gc content pct 44.4 measured

mean read length 151 measured

mean base quality 25.5 measured

adapter content pct 100 measured

duplication rate pct 0 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 0/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 45 measured ×1 0%

adapter content pct 100 measured ×0.5 0%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0