Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA521996

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Lactobacillus crispatus bulk RNA-seq on HiSeq 4000 yields 3.5 billion bases (33.8 million reads) with high quality (97.3% Q30 bases), enabling accurate transcript quantification in this vaginal microbiota member. The excellent quality suits investigation of bacterial gene expression under different conditions or host interactions, relevant to reproductive health and infection studies.

Data type / assay
bulk-RNA-seq
Organism
Lactobacillus crispatus
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
4 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 3542302133 reported
total reads 33789893 reported
n content pct 0.002 measured
pct q20 bases 99.1 measured
pct q30 bases 97.3 measured
gc content pct 40.3 measured
mean read length 144.5 measured
mean base quality 39.8 measured
adapter content pct 0.01 measured
duplication rate pct 77.62 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.3 measured ×1 100%
mean base quality 39.8 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 77.62 measured ×0.4 0%
QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0