Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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PRJNA534176

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

73/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

MiSeq amplicon sequencing of lake water metagenome producing 1.8 million reads and 926 Mb with good quality (92.2% Q20, 81.8% Q30, 45.4% GC). Suitable for freshwater microbial community composition and alpha/beta diversity analysis; relatively modest read depth may limit rare taxon detection and quantitative accuracy.

Data type / assay
amplicon
Organism
lake water metagenome
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
24 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 926301708 reported
total reads 1846435 reported
n content pct 0.012 measured
pct q20 bases 92.2 measured
pct q30 bases 81.8 measured
gc content pct 45.4 measured
mean read length 250.9 measured
mean base quality 33.5 measured
adapter content pct 0.66 measured
duplication rate pct 91.69 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 73/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.8 measured ×1 59%
adapter content pct 0.66 measured ×0.5 100%
QC cost 9 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0