Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA540112

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

77/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk transcriptome sequencing of carrot (Daucus carota) using Illumina HiSeq 2000, yielding 478 million short reads and 144 billion bases with flawless quality metrics (100% Q20 and Q30). Enables comprehensive plant gene expression and metabolic pathway analysis; the 44.6% GC and zero N-content indicate pristine library preparation and sequencing performance. This exceptionally clean dataset is ideal for de novo transcript assembly, novel isoform discovery, and sensitive detection of tissue-specific expression in root vegetables.

Data type / assay
bulk-RNA-seq
Organism
Daucus carota
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
18 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 144067474444 reported
total reads 478746714 reported
n content pct 0.001 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 44.6 measured
mean read length 151 measured
mean base quality 30 measured
adapter content pct 8.35 measured
duplication rate pct 21.49 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 77/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
mean base quality 30 measured ×0.6 33%
adapter content pct 8.35 measured ×0.4 65%
duplication rate pct 21.49 measured ×0.4 100%
QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0