PRJNA540192

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing (16S/ITS-like) in C. elegans. Grade A (100/100) — high-quality amplicon data suitable for reuse. Although duplication is elevated at 30.34% (expected for amplicon, minimal impact on abundance estimates), all quality metrics measured and strong: 91.9% Q30, negligible adapter, and sufficient depth (56k× target coverage, extrapolated) for robust taxonomic profiling.

Data type / assay

amplicon

Organism

Caenorhabditis elegans

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

349 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 112731556787 reported

total reads 383949676 reported

n content pct 0.01 measured

pct q20 bases 96.8 measured

pct q30 bases 91.9 measured

gc content pct 45.1 measured

mean read length 144.5 measured

mean base quality 38.2 measured

adapter content pct 0.02 measured

duplication rate pct 30.34 measured

mean target coverage 56365.8 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.9 measured ×1 100%

adapter content pct 0.02 measured ×0.5 100%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0