Corpus 1,283 assessed · 1,184 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
← Dataset search

PRJNA548649

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Targeted 16S amplicon sequencing of Escherichia coli via Illumina NovaSeq 6000, yielding 1.3 million reads across 391 million bases with good quality (96.2% Q20, 91.9% Q30). Suitable for bacterial strain delineation, population genetics, and pathotype characterization; the 48.2% GC and zero N-content reflect pure culture purity. The moderate read count may limit resolution of rare allelic variants but provides ample depth for core genome SNP calling and phylogenetic placement within E. coli species.

Data type / assay
amplicon
Organism
Escherichia coli
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 391730700 reported
total reads 1305769 reported
n content pct 0 measured
pct q20 bases 96.2 measured
pct q30 bases 91.9 measured
gc content pct 48.2 measured
mean read length 150 measured
mean base quality 35.5 measured
adapter content pct 0 measured
duplication rate pct 96.81 measured
mean target coverage 4257.9 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.9 measured ×1 100%
adapter content pct 0 measured ×0.5 100%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0