PRJNA549801

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Hybrid short+long read whole-genome sequencing from Escherichia coli on MiSeq with ~30 million reads, ~4713× estimated coverage, and good quality (93% Q20, 87.7% Q30), providing exceptional depth for complete genome assembly and structural variation.

Data type / assay

WGS

Organism

Escherichia coli

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

hybrid (short+long)

Files available

FASTQ (raw reads)

N numbers (samples, groups)

216 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 21678348885 reported

total reads 30463210 reported

mean coverage 4712.7 extrapolated

n content pct 0.006 measured

pct q20 bases 93 measured

pct q30 bases 87.7 measured

gc content pct 49.8 measured

mean read length 301 measured

mean base quality 34.9 measured

adapter content pct 41.25 measured

duplication rate pct 5.28 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

mean coverage 4712.7 extrapolated ×1.2 100%

pct q30 bases 87.7 measured ×1 89%

duplication rate pct 5.28 measured ×0.5 100%

adapter content pct 41.25 measured ×0.4 0%

QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0