Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA553700

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

76/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Illumina NovaSeq 6000 RNA-seq of high-GC Pseudomonas aeruginosa with 1.1B reads and exceptional quality (98.7% Q20, 96.2% Q30), ideal for virulence gene expression analysis and biofilm-associated transcriptome studies. Premium quality accommodates challenging GC-rich bacterial transcriptome.

Data type / assay
bulk-RNA-seq
Organism
Pseudomonas aeruginosa
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
14 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 133681549654 reported
total reads 1095750407 reported
n content pct 0.001 measured
pct q20 bases 98.7 measured
pct q30 bases 96.2 measured
gc content pct 60.5 measured
mean read length 122 measured
mean base quality 36.4 measured
adapter content pct 9.38 measured
duplication rate pct 93.01 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 76/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.2 measured ×1 100%
mean base quality 36.4 measured ×0.6 100%
adapter content pct 9.38 measured ×0.4 59%
duplication rate pct 93.01 measured ×0.4 0%
QC cost 58 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0