Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA591860

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 9 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

9 further papers cite this accession but reuse could not be confirmed.

Deep data QC

87/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (100bp, HiSeq, human). Grade B (87/100): high-quality base calls (mean BQ 35.5) severely undercut by duplication rate of 66%—the worst in this cohort. Only ~34% of reads are unique; effective coverage is roughly halved, compromising abundance estimation and rare-transcript discovery despite excellent sequencing chemistry.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
unspecified
Platform
ILLUMINA
N numbers (samples, groups)
23,420 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 11706310384732 reported
total reads 58969654178 reported
n content pct 0.001 measured
pct q20 bases 96.2 measured
pct q30 bases 92 measured
gc content pct 45.4 measured
mean read length 100 measured
mean base quality 35.5 measured
adapter content pct 0.37 measured
duplication rate pct 66.05 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 87/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92 measured ×1 100%
mean base quality 35.5 measured ×0.6 100%
adapter content pct 0.37 measured ×0.4 100%
duplication rate pct 66.05 measured ×0.4 20%
QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it mean score 71