Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA594999

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

34/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Targeted bacterial 16S amplicon sequencing via Illumina MiSeq, yielding 875,000 reads from 439 million total bases with notably lower Q30 accuracy (70.3%) and elevated GC content (56%). Suitable for preliminary microbial community surveys or taxonomic screening; the reduced base quality may necessitate more aggressive QC and chimera-removal steps during amplicon analysis. The sparse read count limits statistical power for rare taxa detection or high-resolution community clustering.

Data type / assay
amplicon
Organism
bacterium
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
42 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 439160142 reported
total reads 874821 reported
n content pct 0 measured
pct q20 bases 78.1 measured
pct q30 bases 70.3 measured
gc content pct 56 measured
mean read length 251 measured
mean base quality 29.2 measured
adapter content pct 0 measured
duplication rate pct 89.71 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 34/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 70.3 measured ×1 2%
adapter content pct 0 measured ×0.5 100%
QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0