PRJNA596996

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

75/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This large human amplicon dataset from MiSeq totals 55.3 billion bases but with elevated N content (0.729%), moderate quality (82.5% Q30), and very high GC content (66%). The large size and high GC suggest challenging target regions.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

3,130 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 55263416341 reported

total reads 233320968 reported

n content pct 0.729 measured

pct q20 bases 88.7 measured

pct q30 bases 82.5 measured

gc content pct 66 measured

mean read length 230.5 measured

mean base quality 33.3 measured

adapter content pct 1.02 measured

duplication rate pct 94.69 measured

mean target coverage 891.3 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 75/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.5 measured ×1 63%

adapter content pct 1.02 measured ×0.5 100%

QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0