Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA605803

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

99/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

16S amplicon sequencing of Mus musculus via Illumina MiniSeq, yielding 9.3 million reads with moderate quality (93% Q20, 89.6% Q30) but notable 0.506% N-content elevation, unusual for amplicon data. Characterizes murine intestinal or other tissue-associated microbiota; the higher N proportion may indicate adapter read-through or contamination and warrants preprocessing inspection. The 48% GC composition is consistent with laboratory mouse microbiota and the read depth permits community profiling and OTU assignment.

Data type / assay
amplicon
Organism
Mus musculus
Instrument
Illumina MiniSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
3 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2791627772 reported
total reads 9277801 reported
n content pct 0.506 measured
pct q20 bases 93 measured
pct q30 bases 89.6 measured
gc content pct 48 measured
mean read length 135.4 measured
mean base quality 35.7 measured
adapter content pct 0.61 measured
duplication rate pct 56.2 measured
mean target coverage 51.7 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 99/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.6 measured ×1 98%
adapter content pct 0.61 measured ×0.5 100%
QC cost 9 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0