PRJNA608281

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

43/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This human gut amplicon dataset from MiSeq totals 3.23 billion bases with low quality (72.9% Q30). The reduced quality may impact reliability of microbiota profiling.

Data type / assay

amplicon

Organism

human gut metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

39 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3228736703 reported

total reads 5372929 reported

n content pct 0 measured

pct q20 bases 85.4 measured

pct q30 bases 72.9 measured

gc content pct 53.2 measured

mean read length 300.7 measured

mean base quality 31.6 measured

adapter content pct 0 measured

duplication rate pct 97.25 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 43/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 72.9 measured ×1 14%

adapter content pct 0 measured ×0.5 100%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0