PRJNA608723

BioProject

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Transcriptome profiling of the model cyanobacterium *Synechocystis* sp. PCC 6803 via Illumina NextSeq 500 with 150 bp reads and high quality (96.2% Q20), enabling gene expression analysis under varied growth conditions. Deep coverage (442M reads) supports sensitive detection of photosynthesis-related transcripts and regulatory responses.

Data type / assay

bulk-RNA-seq

Organism

Synechocystis sp. PCC 6803

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

48 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 66440663550 reported

total reads 442937757 reported

n content pct 0.001 measured

pct q20 bases 96.2 measured

pct q30 bases 94.2 measured

gc content pct 50.5 measured

mean read length 75 measured

mean base quality 34.4 measured

adapter content pct 6.25 measured

duplication rate pct 88.08 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.2 measured ×1 100%

mean base quality 34.4 measured ×0.6 100%

adapter content pct 6.25 measured ×0.4 76%

duplication rate pct 88.08 measured ×0.4 0%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0