PRJNA622393

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

16S amplicon sequencing of mouse gut metagenome on iSeq 100 with ~524k reads and good quality (90.8% Q20, 84% Q30), providing modest coverage from this compact benchtop platform.

Data type / assay

amplicon

Organism

mouse gut metagenome

Instrument

Illumina iSeq 100

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

18 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 157322100 reported

total reads 524407 reported

n content pct 0 measured

pct q20 bases 90.8 measured

pct q30 bases 84 measured

gc content pct 49.6 measured

mean read length 300 measured

mean base quality 33.8 measured

adapter content pct 0.01 measured

duplication rate pct 94.46 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84 measured ×1 70%

adapter content pct 0.01 measured ×0.5 100%

QC cost 3 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0