Corpus 1,278 assessed · 1,179 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA625755

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

16S amplicon survey of rat gut microbiota via Illumina MiniSeq short-read sequencing, generating ~8.5 million reads across 2.6 billion bases with strong base quality (95.5% Q30). The dataset supports community composition and microbial diversity analyses in rodent intestinal microbiomes; note the elevated 55.7% GC content typical of fecal bacterial populations. High sequence reliability (96.4% Q20) makes this suitable for amplicon-based taxonomy and OTU clustering without substantial QC filtering.

Data type / assay
amplicon
Organism
rat gut metagenome
Instrument
Illumina MiniSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
174 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2590717482 reported
total reads 8466397 reported
n content pct 0.007 measured
pct q20 bases 96.4 measured
pct q30 bases 95.5 measured
gc content pct 55.7 measured
mean read length 153 measured
mean base quality 35.9 measured
adapter content pct 0 measured
duplication rate pct 94.9 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.5 measured ×1 100%
adapter content pct 0 measured ×0.5 100%
QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0