Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA631961

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

51/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This amplicon dataset from synthetic constructs on MiSeq totals 2.95 billion bases with moderate quality (85.3% Q30). The controlled sample design enables validation of amplicon sequencing performance on engineered targets.

Data type / assay
amplicon
Organism
synthetic construct
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
7 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2950335600 reported
total reads 4917226 reported
n content pct 0 measured
pct q20 bases 89.2 measured
pct q30 bases 85.3 measured
gc content pct 46 measured
mean read length 300 measured
mean base quality 34.2 measured
adapter content pct 36.26 measured
duplication rate pct 68 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 51/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.3 measured ×1 77%
adapter content pct 36.26 measured ×0.5 0%
QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0