PRJNA636273

BioProject

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Tobacco (*Nicotiana tabacum*) transcriptome sequencing via Illumina HiSeq 2500 with modest read length (50.8 bp) and good quality (96% Q20), suitable for gene expression quantification and transcript discovery. Relatively short reads complicate mapping to complex plant genomes with numerous paralogs and repetitive elements.

Data type / assay

bulk-RNA-seq

Organism

Nicotiana tabacum

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

4 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 10171798767 reported

total reads 199868951 reported

n content pct 0.088 measured

pct q20 bases 96 measured

pct q30 bases 91.5 measured

gc content pct 44.3 measured

mean read length 59.4 measured

mean base quality 35.7 measured

adapter content pct 0.01 measured

duplication rate pct 30.99 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.5 measured ×1 100%

mean base quality 35.7 measured ×0.6 100%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 30.99 measured ×0.4 98%

QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0