Corpus 1,278 assessed · 1,179 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA642411

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

90/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Targeted amplicon survey of Escherichia coli via Illumina MiSeq with hybrid (short + long) read sequencing, delivering 403 million reads (118 billion bases) with good quality (94.6% Q20, 87.1% Q30) and zero N content. The hybrid read strategy enables detection of large insertion sequences, plasmid architectures, and pathogenicity island organization in pathogenic E. coli strains; the 56.5% GC and long-read support resolve complex loci missed by short-read-only approaches. Supports virulence profiling and antibiotic resistance gene identification in clinical isolates via comprehensive genomic context.

Data type / assay
amplicon
Organism
Escherichia coli
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
hybrid (short+long)
Files available
FASTQ (raw reads)
N numbers (samples, groups)
210 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 118259673918 reported
total reads 403016176 reported
n content pct 0 measured
pct q20 bases 94.6 measured
pct q30 bases 87.1 measured
gc content pct 56.5 measured
mean read length 270 measured
mean base quality 34.9 measured
adapter content pct 0.57 measured
duplication rate pct 97.33 measured
mean target coverage 1285431.2 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 90/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 87.1 measured ×1 86%
adapter content pct 0.57 measured ×0.5 100%
QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0