Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA649277

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Whole-genome sequencing of Escherichia coli B str. REL606 via Illumina MiSeq, yielding 30 million short reads and 16.6 billion bases with moderate quality (89% Q20, 77.9% Q30) and the expected 50.4% GC. Enables strain genome assembly, mutation discovery, and population genetics in this canonical laboratory E. coli reference strain; the notably lower Q30 compared to HiSeq platforms may necessitate more aggressive quality filtering or read overlap assembly strategies. Despite reduced base quality, the read count provides sufficient coverage for core genome SNP calling and chromosomal rearrangement detection.

Data type / assay
WGS
Organism
Escherichia coli B str. REL606
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
64 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 16643978293 reported
total reads 30055584 reported
n content pct 0.012 measured
pct q20 bases 89 measured
pct q30 bases 77.9 measured
gc content pct 50.4 measured
mean read length 276.5 measured
mean base quality 32.8 measured
adapter content pct 0 measured
duplication rate pct 4.42 measured
How this grade was computed
Weighted mean of 3 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 77.9 measured ×1 40%
duplication rate pct 4.42 measured ×0.5 100%
adapter content pct 0 measured ×0.4 100%
QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0