Corpus 1,278 assessed · 1,179 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA655492

BioProject first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

16S amplicon profiling of Drosophila melanogaster-associated microbiota via Illumina MiSeq, producing 3.9 million reads with moderate quality (92.6% Q20, 92.1% Q30) but notably elevated 6.315% N-content, an anomaly for amplicon sequencing. Characterizes fruit fly holobiont microbial symbionts; the high N proportion suggests possible adapter contamination or incomplete trimming and requires preprocessing validation. The 56.8% GC and read depth support community composition analysis if N-rich reads are filtered, but warrants investigation of library preparation quality.

Data type / assay
amplicon
Organism
Drosophila melanogaster
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
61 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1637583301 reported
total reads 3932912 reported
n content pct 6.315 measured
pct q20 bases 92.6 measured
pct q30 bases 92.1 measured
gc content pct 56.8 measured
mean read length 118.6 measured
mean base quality 35 measured
adapter content pct 0.68 measured
duplication rate pct 98.98 measured
mean target coverage 584.9 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.1 measured ×1 100%
adapter content pct 0.68 measured ×0.5 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0