Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJNA661613

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Targeted-capture (WES-like) sequencing of metagenome via Illumina NextSeq 550, producing 306 million short reads (46.5 billion bases) with good quality (93.9% Q20, 92.1% Q30) and low N-content (0.022%). Enables functional metagenomic profiling with reduced off-target sequencing compared to whole-metagenome approaches; the 49% GC reflects diverse microbial composition. The targeted enrichment strategy provides higher depth-of-coverage per target gene compared to shotgun metagenomics, supporting sensitive functional gene discovery and abundance quantification in complex environmental samples.

Data type / assay
WES
Organism
metagenome
Instrument
NextSeq 550
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
18 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 46534180520 reported
total reads 306407694 reported
n content pct 0.022 measured
pct q20 bases 93.9 measured
pct q30 bases 92.1 measured
gc content pct 49 measured
mean read length 71.7 measured
mean base quality 34 measured
adapter content pct 0 measured
duplication rate pct 48.35 measured
How this grade was computed
Weighted mean of 3 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published WES thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.1 measured ×1 100%
duplication rate pct 48.35 measured ×0.5 0%
adapter content pct 0 measured ×0.4 100%
QC cost 11 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0