Corpus 1,278 assessed · 1,179 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA664762

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Amplicon survey of marine metagenome via AB 3730xL capillary sequencing (Sanger-legacy platform), generating 883 million reads (276 billion bases) with good quality (96.5% Q20, 90.7% Q30) despite capillary-sequencing technology. Represents a large-scale marine microbial profiling effort; the 52.2% GC and high read count enable robust community composition surveys, though capillary-based amplicon sequencing is computationally distinct from Illumina/NGS workflows. This legacy dataset provides historical baseline for marine microbial communities and demonstrates feasibility of large-scale capillary-based metabarcoding, now superseded by higher-throughput platforms.

Data type / assay
amplicon
Organism
marine metagenome
Instrument
AB 3730xL Genetic Analyzer
Platform
CAPILLARY
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1,702 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 276586651347 reported
total reads 883528007 reported
n content pct 0.001 measured
pct q20 bases 96.5 measured
pct q30 bases 90.7 measured
gc content pct 52.2 measured
mean read length 300.1 measured
mean base quality 35.7 measured
adapter content pct 0 measured
duplication rate pct 94.81 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.7 measured ×1 100%
adapter content pct 0 measured ×0.5 100%
QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0