PRJNA685637

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina NextSeq 500 RNA-seq of human tissue with 166.3M reads and reported perfect Q-scores (100% Q20/Q30—likely a capped quality metric), moderate depth sufficient for gene-level abundance quantification. Verify actual quality metrics before relying on reported perfect scores.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

71 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 12316364234 reported

total reads 166345832 reported

n content pct 0.089 measured

pct q20 bases 100 measured

pct q30 bases 100 measured

gc content pct 52.1 measured

mean read length 29.5 measured

mean base quality 30 measured

adapter content pct 0 measured

duplication rate pct 0.04 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%

mean base quality 30 measured ×0.6 33%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 0.04 measured ×0.4 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0