PRJNA687536

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

11/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina HiSeq 2500 WGS with severe technical issues: 24.9% N-content and low base quality (68.1% Q20, 63.8% Q30) significantly limit assembly and SNP detection. Investigate sequencing chemistry failure before attempting analysis; likely unsuitable for high-confidence variant calling.

Data type / assay

WGS

Organism

metagenome

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

46 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 73756306360 reported

total reads 244226180 reported

n content pct 24.919 measured

pct q20 bases 68.1 measured

pct q30 bases 63.8 measured

gc content pct 32.9 measured

mean read length 151 measured

mean base quality 26.7 measured

adapter content pct 44.51 measured

duplication rate pct 26.92 measured

How this grade was computed

Weighted mean of 3 scored metric(s) → 11/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published WGS thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 63.8 measured ×1 0%

duplication rate pct 26.92 measured ×0.5 41%

adapter content pct 44.51 measured ×0.4 0%

QC cost 43 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0