Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA714981

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

19/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Illumina MiSeq amplicon of model E. coli K-12 strain with 4.9M reads and moderate quality (88.2% Q20, 75.7% Q30), suitable for targeted gene expression or SNP analysis in laboratory isolates. Limited depth and lower Q30 suggest preliminary or validation studies.

Data type / assay
amplicon
Organism
Escherichia coli K-12
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
2 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 2444208000 reported
total reads 4888416 reported
n content pct 0.003 measured
pct q20 bases 88.2 measured
pct q30 bases 75.7 measured
gc content pct 54.2 measured
mean read length 250 measured
mean base quality 32.4 measured
adapter content pct 78.99 measured
duplication rate pct 70.79 measured
How this grade was computed
Weighted mean of 2 scored metric(s) → 19/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 75.7 measured ×1 28%
adapter content pct 78.99 measured ×0.5 0%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0