PRJNA715507

BioProject first seen 2022

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mus musculus bulk RNA-seq via NovaSeq 6000 from five samples totaling 343 billion bases (97.7% Q20), delivering exceptional transcriptome depth suitable for detecting subtle expression differences and low-abundance transcripts; outstanding read quality (97.7% Q30) ensures high-confidence quantitation.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina NovaSeq 6000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

5 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 343663510934 reported

total reads 2937133345 reported

n content pct 0.008 measured

pct q20 bases 97.7 measured

pct q30 bases 97.7 measured

gc content pct 45.3 measured

mean read length 91 measured

mean base quality 29.4 measured

adapter content pct 0.03 measured

duplication rate pct 35.01 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.7 measured ×1 100%

mean base quality 29.4 measured ×0.6 23%

adapter content pct 0.03 measured ×0.4 100%

duplication rate pct 35.01 measured ×0.4 89%

QC cost 48 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0