PRJNA720523

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mus musculus amplicon sequencing via MiSeq from 42 samples totaling 1.4 billion bases (87.9% Q20), supporting targeted genomic region profiling; reduced Q30 rate (80.4%) indicates some quality trade-offs for high sample throughput.

Data type / assay

amplicon

Organism

Mus musculus

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

42 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 1412073290 reported

total reads 2812895 reported

n content pct 0 measured

pct q20 bases 87.9 measured

pct q30 bases 80.4 measured

gc content pct 52.8 measured

mean read length 251 measured

mean base quality 33.2 measured

adapter content pct 0 measured

duplication rate pct 90.93 measured

mean target coverage 26.1 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.4 measured ×1 52%

adapter content pct 0 measured ×0.5 100%

QC cost 2 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0