Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJNA731531

BioProject first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Oxford Nanopore MinION long-read RNA-seq of E. coli with 9.9M reads and low per-read accuracy (38% Q20, 11.8% Q30), enabling full-length transcript recovery despite minimal short-read equivalence. Valuable for resolving prokaryotic transcriptome complexity and junction-spanning isoforms.

Data type / assay
bulk-RNA-seq
Organism
Escherichia coli
Instrument
MinION
Platform
OXFORD_NANOPORE
Read type
long-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
10 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 5686941260 reported
total reads 9856223 reported
n content pct 0 measured
pct q20 bases 38 measured
pct q30 bases 11.8 measured
gc content pct 47.9 measured
mean read length 597 measured
mean base quality 16.3 measured
adapter content pct 0 measured
duplication rate pct 0.02 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 11.8 measured ×1 0%
mean base quality 16.3 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 0.02 measured ×0.4 100%
QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0